多肽CRDMO平台
目录肽
大奖888生化目录肽版块全新呈现，致力于打造全球最全面的多肽供应平台！我们专业的学术团队将时刻追踪最新最热的研究动向，帮助您发现实际需求，并以最高质量标准提供您所需要的产品。我们将我们将不定期更新产品列表，为您最大限度地节约科研时间，敬请广大客户朋友关注！
RENI-013
Renin FRET Substrate I
DABCYL-gama-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS
DABCYL-gama-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS
Size：1
P1(RMB)：851
MW：1797.9
One letter sequence：DABCYL-g-Abu-IHPFHLVIHT-EDANS
Molecular Formula：
Description：DABCYL-GABA-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS (also called Renin Substrate I in some literature) contains a renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen. Cleavage of the substrate occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen. This fluorogenic peptide substrate is used to continuously measure the proteolytic activity of human renin. The assay relies upon FRET-mediated, intramolecular fluorescence quenching that occurs in the intact peptide substrate. Efficient fluorescence quenching occurs as a result of favorable energetic overlap of the EDANS excited state and the DABCYL absorption, and the relatively long excited state lifetime of the EDANS fluorophore. Cleavage of the substrate by renin liberates the peptidyl-EDANS fragment from proximity with the DABCYL acceptor, restoring the fluorescence of the EDANS moiety. This leads to a time-dependent increase in fluorescence intensity, directly related to the extent of substrate cleavage. The kinetics of renin-catalyzed hydrolysis of this substrate is shown to be consistent with a simple substrate inhibition model with a substrate Km approximately equal to 1.5 mM at physiological pH. The substrate can detect renin as low as 30 ng/mL after an incubation of only 3-5 min.
Literature Reference：Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9, Nakamura N, et al. (1991). Identification of the active site of human renin with use of new fluorogenic peptides. J Biochem (Tokyo) 109, 741-5, Murakami K, et al. (1981). New fluorogenic substrates for renin. Anal Biochem 110, 232-9.
Cas：
P1(RMB)：851
MW：1797.9
One letter sequence：DABCYL-g-Abu-IHPFHLVIHT-EDANS
Molecular Formula：
Description：DABCYL-GABA-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS (also called Renin Substrate I in some literature) contains a renin cleavage site that occurs in the N-terminal peptide of human angiotensinogen. Cleavage of the substrate occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen. This fluorogenic peptide substrate is used to continuously measure the proteolytic activity of human renin. The assay relies upon FRET-mediated, intramolecular fluorescence quenching that occurs in the intact peptide substrate. Efficient fluorescence quenching occurs as a result of favorable energetic overlap of the EDANS excited state and the DABCYL absorption, and the relatively long excited state lifetime of the EDANS fluorophore. Cleavage of the substrate by renin liberates the peptidyl-EDANS fragment from proximity with the DABCYL acceptor, restoring the fluorescence of the EDANS moiety. This leads to a time-dependent increase in fluorescence intensity, directly related to the extent of substrate cleavage. The kinetics of renin-catalyzed hydrolysis of this substrate is shown to be consistent with a simple substrate inhibition model with a substrate Km approximately equal to 1.5 mM at physiological pH. The substrate can detect renin as low as 30 ng/mL after an incubation of only 3-5 min.
Literature Reference：Wang GT, et al. (1993). A continuous fluorescence assay of renin activity. Anal Biochem 210, 351-9, Nakamura N, et al. (1991). Identification of the active site of human renin with use of new fluorogenic peptides. J Biochem (Tokyo) 109, 741-5, Murakami K, et al. (1981). New fluorogenic substrates for renin. Anal Biochem 110, 232-9.
Cas：
